[RCAC Workshop] RNA-seq in practice: A one-day hands-on workshop on RCAC systems
📅 Date: Thursday, November 20, 2025
⏰ Time: 9:00 AM – 4:00 PM (ET)
💻 Location: SC 179 [in-person]
🏫 Instructors: Arun Seetharam and Michael Carlson
Who Should Attend
Researchers, graduate students, and postdocs who want to perform RNA sequencing (RNA-seq) analysis on Purdue’s high-performance computing (HPC) systems. Participants should have basic command-line familiarity but no prior experience with RNA-seq analysis is required. You do not need an existing RCAC account; workshop access will be arranged for attendees.
What You’ll Learn
This hands-on workshop introduces participants to the complete RNA-seq analysis workflow, from accessing RCAC systems to generating differential expression results. Emphasis will be on practical execution and reproducibility, with minimal theoretical background.
By the End of the Session, You’ll
- Access and manage your environment on RCAC clusters.
- Perform pre-alignment quality control and reference indexing.
- Align RNA-seq reads, evaluate mapping quality, and visualize results.
- Quantify transcript abundance and identify differentially expressed genes.
- Use Kallisto for lightweight, reference-free quantification.
Level
Intermediate, suitable for those comfortable with the Linux command line who wish to gain practical experience in running RNA-seq analyses on an HPC cluster.
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