Purdue University

Commands

• last-dotplot
• last-map-probs
• last-merge-batches
• last-pair-probs
• last-postmask
• last-split
• last-split5
• last-train
• lastal
• lastal5
• lastdb
• lastdb5

Module

You can load the modules by:

module load biocontainers
module load last

Example job

Using #!/bin/sh -l as shebang in the slurm job script will cause the failure of some biocontainer modules. Please use #!/bin/bash instead.

To run Last on our clusters:

#!/bin/bash
#SBATCH -A myallocation     # Allocation name 
#SBATCH -t 1:00:00
#SBATCH -N 1
#SBATCH -n 1
#SBATCH --job-name=last
#SBATCH --mail-type=FAIL,BEGIN,END
#SBATCH --error=%x-%J-%u.err
#SBATCH --output=%x-%J-%u.out

module --force purge
ml biocontainers last

lastdb humdb humanMito.fa
lastal humdb fuguMito.fa > myalns.maf