Software: Model-based Analysis of Genome-wide CRISPR-Cas9 Knockout (MAGeCK)
Description
Model-based Analysis of Genome-wide CRISPR-Cas9 Knockout (MAGeCK) is a computational tool to identify important genes from the recent genome-scale CRISPR-Cas9 knockout screens (or GeCKO) technology.
Available Versions
Unspecified: | 0.5.9.5 |
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Docker hub: https://hub.docker.com/r/davidliwei/mageck
Home page: https://bitbucket.org/liulab/mageck/src/master/
Commands
- mageck
- mageckGSEA
- RRA
Module
You can load the modules by:
module load biocontainers
module load mageck
Example job
Using
#!/bin/sh -l
as shebang in the slurm job script will cause the failure of some biocontainer modules. Please use#!/bin/bash
instead.
To run mageck on our clusters:
#!/bin/bash
#SBATCH -A myallocation # Allocation name
#SBATCH -t 1:00:00
#SBATCH -N 1
#SBATCH -n 1
#SBATCH --job-name=mageck
#SBATCH --mail-type=FAIL,BEGIN,END
#SBATCH --error=%x-%J-%u.err
#SBATCH --output=%x-%J-%u.out
module --force purge
ml biocontainers mageck
mageck count -l library.txt -n demo \
--sample-label L1,CTRL \
--fastq test1.fastq test2.fastq
mageck test -k demo.count.txt \
-t L1 -c CTRL -n demo