Purdue University

Commands

• vsearch

Module

You can load the modules by:

module load biocontainers
module load vsearch

Example job

Using #!/bin/sh -l as shebang in the slurm job script will cause the failure of some biocontainer modules. Please use #!/bin/bash instead.

To run Vsearch on our clusters:

#!/bin/bash
#SBATCH -A myallocation     # Allocation name 
#SBATCH -t 1:00:00
#SBATCH -N 1
#SBATCH -n 1
#SBATCH --job-name=vsearch
#SBATCH --mail-type=FAIL,BEGIN,END
#SBATCH --error=%x-%J-%u.err
#SBATCH --output=%x-%J-%u.out

module --force purge
ml biocontainers vsearch

vsearch -sintax SRR8723605_merged.fasta -db rdp_16s_v16_sp.fa \
    -tabbedout SRR8723605_out.txt -strand both -sintax_cutoff 0.5