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bismark

Link to section 'Description' of 'bismark' Description

A tool to map bisulfite converted sequence reads and determine cytosine methylation states

Link to section 'Versions' of 'bismark' Versions

  • Anvil: 0.23.0

Link to section 'Commands' of 'bismark' Commands

  • bam2nuc
  • bismark
  • bismark2bedGraph
  • bismark2report
  • bismark2summary
  • bismark_genome_preparation
  • bismark_methylation_extractor
  • coverage2cytosine
  • deduplicate_bismark
  • filter_non_conversion
  • NOMe_filtering

Link to section 'Module' of 'bismark' Module

You can load the modules by:

module load bismark

Link to section 'Example job' of 'bismark' Example job

To run Bismark on our clusters:

#!/bin/bash
#SBATCH -A myAllocation     # Allocation name 
#SBATCH -t 1:00:00
#SBATCH -N 1
#SBATCH -n 12
#SBATCH --job-name=bismark
#SBATCH --mail-type=FAIL,BEGIN,END
#SBATCH --error=%x-%J-%u.err
#SBATCH --output=%x-%J-%u.out

module load bismark

bismark_genome_preparation --bowtie2 data/ref_genome

bismark --multicore 12 --genome data/ref_genome seq.fastq
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