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tophat

Link to section 'Description' of 'tophat' Description

Spliced read mapper for RNA-Seq.

Link to section 'Versions' of 'tophat' Versions

  • Anvil: 2.1.2

Link to section 'Commands' of 'tophat' Commands

  • bam2fastx
  • bam_merge
  • bed_to_juncs
  • contig_to_chr_coords
  • fix_map_ordering
  • gtf_juncs
  • gtf_to_fasta
  • juncs_db
  • long_spanning_reads
  • map2gtf
  • prep_reads
  • sam_juncs
  • samtools_0.1.18
  • segment_juncs
  • sra_to_solid
  • tophat
  • tophat2
  • tophat-fusion-post
  • tophat_reports

Link to section 'Module' of 'tophat' Module

You can load the modules by:

module load tophat

Link to section 'Example job' of 'tophat' Example job

To run TopHat on our clusters:

#!/bin/bash
#SBATCH -A myallocation     # Allocation name 
#SBATCH -t 1:00:00
#SBATCH -N 1
#SBATCH -n 1
#SBATCH --job-name=tophat
#SBATCH --mail-type=FAIL,BEGIN,END
#SBATCH --error=%x-%J-%u.err
#SBATCH --output=%x-%J-%u.out

module load tophat

tophat -r 20 test_ref reads_1.fq reads_2.fq
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