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deeptools

Link to section 'Introduction' of 'deeptools' Introduction

DeepTools is a collection of user-friendly tools for normalization and visualization of deep-sequencing data.

For more information, please check its website: https://biocontainers.pro/tools/deeptools and its home page on Github.

Link to section 'Versions' of 'deeptools' Versions

  • 3.5.1-py

Link to section 'Commands' of 'deeptools' Commands

  • alignmentSieve
  • bamCompare
  • bamCoverage
  • bamPEFragmentSize
  • bigwigCompare
  • computeGCBias
  • computeMatrix
  • computeMatrixOperations
  • correctGCBias
  • deeptools
  • estimateReadFiltering
  • estimateScaleFactor
  • multiBamSummary
  • multiBigwigSummary
  • plotCorrelation
  • plotCoverage
  • plotEnrichment
  • plotFingerprint
  • plotHeatmap
  • plotPCA
  • plotProfile

Link to section 'Module' of 'deeptools' Module

You can load the modules by:

module load biocontainers
module load deeptools

Link to section 'Example job' of 'deeptools' Example job

Using #!/bin/sh -l as shebang in the slurm job script will cause the failure of some biocontainer modules. Please use #!/bin/bash instead.

To run DeepTools on our clusters:

#!/bin/bash
#SBATCH -A myallocation     # Allocation name 
#SBATCH -t 1:00:00
#SBATCH -N 1
#SBATCH -n 4
#SBATCH --job-name=deeptools
#SBATCH --mail-type=FAIL,BEGIN,END
#SBATCH --error=%x-%J-%u.err
#SBATCH --output=%x-%J-%u.out

module --force purge
ml biocontainers deeptools

bamCoverage  --normalizeUsing CPM -p 32  \
     --effectiveGenomeSize  11000000  \
     -b WT_coord_sorted.bam  \
     -o WT_coord_sorted.bw  
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