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ngs-bits

Link to section 'Introduction' of 'ngs-bits' Introduction

Ngs-bits - Short-read sequencing tools.

For more information, please check its website: https://biocontainers.pro/tools/ngs-bits and its home page on Github.

Link to section 'Versions' of 'ngs-bits' Versions

  • 2022_04

Link to section 'Commands' of 'ngs-bits' Commands

  • SampleAncestry
  • SampleDiff
  • SampleGender
  • SampleOverview
  • SampleSimilarity
  • SeqPurge
  • CnvHunter
  • RohHunter
  • UpdHunter
  • CfDnaQC
  • MappingQC
  • NGSDImportQC
  • ReadQC
  • SomaticQC
  • VariantQC
  • TrioMaternalContamination
  • BamCleanHaloplex
  • BamClipOverlap
  • BamDownsample
  • BamFilter
  • BamToFastq
  • BedAdd
  • BedAnnotateFreq
  • BedAnnotateFromBed
  • BedAnnotateGC
  • BedAnnotateGenes
  • BedChunk
  • BedCoverage
  • BedExtend
  • BedGeneOverlap
  • BedHighCoverage
  • BedInfo
  • BedIntersect
  • BedLiftOver
  • BedLowCoverage
  • BedMerge
  • BedReadCount
  • BedShrink
  • BedSort
  • BedSubtract
  • BedToFasta
  • BedpeAnnotateBreakpointDensity
  • BedpeAnnotateCnvOverlap
  • BedpeAnnotateCounts
  • BedpeAnnotateFromBed
  • BedpeFilter
  • BedpeGeneAnnotation
  • BedpeSort
  • BedpeToBed
  • FastqAddBarcode
  • FastqConcat
  • FastqConvert
  • FastqDownsample
  • FastqExtract
  • FastqExtractBarcode
  • FastqExtractUMI
  • FastqFormat
  • FastqList
  • FastqMidParser
  • FastqToFasta
  • FastqTrim
  • VcfAnnotateFromBed
  • VcfAnnotateFromBigWig
  • VcfAnnotateFromVcf
  • VcfBreakMulti
  • VcfCalculatePRS
  • VcfCheck
  • VcfExtractSamples
  • VcfFilter
  • VcfLeftNormalize
  • VcfSort
  • VcfStreamSort
  • VcfToBedpe
  • VcfToTsv
  • SvFilterAnnotations
  • NGSDExportGenes
  • GenePrioritization
  • GenesToApproved
  • GenesToBed
  • GraphStringDb
  • PhenotypeSubtree
  • PhenotypesToGenes
  • PERsim
  • FastaInfo

Link to section 'Module' of 'ngs-bits' Module

You can load the modules by:

module load biocontainers
module load ngs-bits

Link to section 'Example job' of 'ngs-bits' Example job

Using #!/bin/sh -l as shebang in the slurm job script will cause the failure of some biocontainer modules. Please use #!/bin/bash instead.

To run Ngs-bits on our clusters:

#!/bin/bash
#SBATCH -A myallocation     # Allocation name 
#SBATCH -t 1:00:00
#SBATCH -N 1
#SBATCH -n 1
#SBATCH --job-name=ngs-bits
#SBATCH --mail-type=FAIL,BEGIN,END
#SBATCH --error=%x-%J-%u.err
#SBATCH --output=%x-%J-%u.out

module --force purge
ml biocontainers ngs-bits

SeqPurge -in1 input1_1.fastq input2_1.fastq \
     -in2 input2_2.fastq input2_2.fastq \
     -out1 R1.fastq.gz -out2 R2.fastq.gz
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