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last

Link to section 'Introduction' of 'last' Introduction

Last is used to find & align related regions of sequences.

For more information, please check its website: https://biocontainers.pro/tools/last and its home page on Gitlab.

Link to section 'Versions' of 'last' Versions

  • 1268
  • 1356
  • 1411
  • 1418

Link to section 'Commands' of 'last' Commands

  • last-dotplot
  • last-map-probs
  • last-merge-batches
  • last-pair-probs
  • last-postmask
  • last-split
  • last-split5
  • last-train
  • lastal
  • lastal5
  • lastdb
  • lastdb5

Link to section 'Module' of 'last' Module

You can load the modules by:

module load biocontainers
module load last

Link to section 'Example job' of 'last' Example job

Using #!/bin/sh -l as shebang in the slurm job script will cause the failure of some biocontainer modules. Please use #!/bin/bash instead.

To run Last on our clusters:

#!/bin/bash
#SBATCH -A myallocation     # Allocation name 
#SBATCH -t 1:00:00
#SBATCH -N 1
#SBATCH -n 1
#SBATCH --job-name=last
#SBATCH --mail-type=FAIL,BEGIN,END
#SBATCH --error=%x-%J-%u.err
#SBATCH --output=%x-%J-%u.out

module --force purge
ml biocontainers last

lastdb humdb humanMito.fa
lastal humdb fuguMito.fa > myalns.maf
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