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rasusa

Link to section 'Introduction' of 'rasusa' Introduction

Rasusa: Randomly subsample sequencing reads to a specified coverage.

Docker hub: https://hub.docker.com/r/staphb/rasusa
Home page: https://github.com/mbhall88/rasusa

Link to section 'Versions' of 'rasusa' Versions

  • 0.6.0
  • 0.7.0

Link to section 'Commands' of 'rasusa' Commands

  • rasusa

Link to section 'Module' of 'rasusa' Module

You can load the modules by:

module load biocontainers
module load rasusa

Link to section 'Example job' of 'rasusa' Example job

Using #!/bin/sh -l as shebang in the slurm job script will cause the failure of some biocontainer modules. Please use #!/bin/bash instead.

To run rasusa on our clusters:

#!/bin/bash
#SBATCH -A myallocation     # Allocation name
#SBATCH -t 1:00:00
#SBATCH -N 1
#SBATCH -n 1
#SBATCH --job-name=rasusa
#SBATCH --mail-type=FAIL,BEGIN,END
#SBATCH --error=%x-%J-%u.err
#SBATCH --output=%x-%J-%u.out

module --force purge
ml biocontainers rasusa

rasusa -i seq_1.fq -i seq_2.fq  \
    --coverage 100 --genome-size 35mb  \
    -o out.r1.fq -o out.r2.fq
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