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trust4

Link to section 'Introduction' of 'trust4' Introduction

Tcr Receptor Utilities for Solid Tissue (TRUST) is a computational tool to analyze TCR and BCR sequences using unselected RNA sequencing data, profiled from solid tissues, including tumors.

BioContainers: https://biocontainers.pro/tools/trust4
Home page: https://github.com/liulab-dfci/TRUST4

Link to section 'Versions' of 'trust4' Versions

  • 1.0.7

Link to section 'Commands' of 'trust4' Commands

  • run-trust4
  • BuildDatabaseFa.pl
  • BuildImgtAnnot.pl
  • trust-airr.pl
  • trust-barcoderep.pl
  • trust-simplerep.pl
  • trust-smartseq.pl

Link to section 'Module' of 'trust4' Module

You can load the modules by:

module load biocontainers
module load trust4

Link to section 'Example job' of 'trust4' Example job

Using #!/bin/sh -l as shebang in the slurm job script will cause the failure of some biocontainer modules. Please use #!/bin/bash instead.

To run trust4 on our clusters:

#!/bin/bash
#SBATCH -A myallocation     # Allocation name
#SBATCH -t 1:00:00
#SBATCH -N 1
#SBATCH -n 1
#SBATCH --job-name=trust4
#SBATCH --mail-type=FAIL,BEGIN,END
#SBATCH --error=%x-%J-%u.err
#SBATCH --output=%x-%J-%u.out

module --force purge
ml biocontainers trust4

run-trust4 -b mapped.bam -f hg38_bcrtcr.fa --ref human_IMGT+C.fa
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