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snpgenie

Link to section 'Introduction' of 'snpgenie' Introduction

Snpgenie is a collection of Perl scripts for estimating πN/πS, dN/dS, and gene diversity from next-generation sequencing (NGS) single-nucleotide polymorphism (SNP) variant data.

For more information, please check its website: https://biocontainers.pro/tools/snpgenie and its home page on Github.

Link to section 'Versions' of 'snpgenie' Versions

  • 1.0

Link to section 'Commands' of 'snpgenie' Commands

  • fasta2revcom.pl
  • gtf2revcom.pl
  • snpgenie.pl
  • snpgenie_between_group.pl
  • snpgenie_between_group_processor.pl
  • snpgenie_within_group.pl
  • snpgenie_within_group_processor.pl
  • vcf2revcom.pl

Link to section 'Module' of 'snpgenie' Module

You can load the modules by:

module load biocontainers
module load snpgenie

Link to section 'Example job' of 'snpgenie' Example job

Using #!/bin/sh -l as shebang in the slurm job script will cause the failure of some biocontainer modules. Please use #!/bin/bash instead.

To run Snpgenie on our clusters:

#!/bin/bash
#SBATCH -A myallocation     # Allocation name 
#SBATCH -t 1:00:00
#SBATCH -N 1
#SBATCH -n 1
#SBATCH --job-name=snpgenie
#SBATCH --mail-type=FAIL,BEGIN,END
#SBATCH --error=%x-%J-%u.err
#SBATCH --output=%x-%J-%u.out

module --force purge
ml biocontainers snpgenie

snpgenie.pl --minfreq=0.01 --snpreport=CLC_SNP_EXAMPLE.txt \
    --fastafile=REFERENCE_EXAMPLE.fasta --gtffile=CDS_EXAMPLE.gtf
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