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trinity

Link to section 'Introduction' of 'trinity' Introduction

Trinity assembles transcript sequences from Illumina RNA-Seq data.

For more information, please check its website: https://biocontainers.pro/tools/trinity and its home page on Github.

Link to section 'Versions' of 'trinity' Versions

  • 2.12.0
  • 2.13.2
  • 2.14.0
  • 2.15.0

Link to section 'Commands' of 'trinity' Commands

  • Trinity
  • TrinityStats.pl
  • Trinity_gene_splice_modeler.py
  • ace2sam
  • align_and_estimate_abundance.pl
  • analyze_blastPlus_topHit_coverage.pl
  • analyze_diff_expr.pl
  • blast2sam.pl
  • bowtie
  • bowtie2
  • bowtie2-build
  • bowtie2-inspect
  • bowtie2sam.pl
  • contig_ExN50_statistic.pl
  • define_clusters_by_cutting_tree.pl
  • export2sam.pl
  • extract_supertranscript_from_reference.py
  • filter_low_expr_transcripts.pl
  • get_Trinity_gene_to_trans_map.pl
  • insilico_read_normalization.pl
  • interpolate_sam.pl
  • jellyfish
  • novo2sam.pl
  • retrieve_sequences_from_fasta.pl
  • run_DE_analysis.pl
  • sam2vcf.pl
  • samtools
  • samtools.pl
  • seq_cache_populate.pl
  • seqtk-trinity
  • sift_bam_max_cov.pl
  • soap2sam.pl
  • tabix
  • trimmomatic
  • wgsim
  • wgsim_eval.pl
  • zoom2sam.pl

Link to section 'Module' of 'trinity' Module

You can load the modules by:

module load biocontainers
module load trinity

Link to section 'Example job' of 'trinity' Example job

Using #!/bin/sh -l as shebang in the slurm job script will cause the failure of some biocontainer modules. Please use #!/bin/bash instead.

To run Trinity on our clusters:

#!/bin/bash
#SBATCH -A myallocation     # Allocation name 
#SBATCH -t 1:00:00
#SBATCH -N 1
#SBATCH -n 6
#SBATCH --job-name=trinity
#SBATCH --mail-type=FAIL,BEGIN,END
#SBATCH --error=%x-%J-%u.err
#SBATCH --output=%x-%J-%u.out

module --force purge
ml biocontainers trinity

Trinity --seqType fq --left reads_1.fq --right reads_2.fq \
    --CPU 6 --max_memory 20G 
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