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sra-tools

SRA-Toolkit is a collection of tools and libraries for using data in the INSDC Sequence Read Archives. Its detailed documentation can be found in https://trace.ncbi.nlm.nih.gov/Traces/sra/sra.cgi?view=toolkit_doc.

Link to section 'Versions' of 'sra-tools' Versions

  • 2.11.0-pl5262

Link to section 'Commands' of 'sra-tools' Commands

  • abi-dump
  • align-cache
  • align-info
  • bam-load
  • cache-mgr
  • cg-load
  • fasterq-dump
  • fasterq-dump-orig
  • fastq-dump
  • fastq-dump-orig
  • illumina-dump
  • kar
  • kdbmeta
  • kget
  • latf-load
  • md5cp
  • prefetch
  • prefetch-orig
  • rcexplain
  • read-filter-redact
  • sam-dump
  • sam-dump-orig
  • sff-dump
  • sra-pileup
  • sra-pileup-orig
  • sra-sort
  • sra-sort-cg
  • sra-stat
  • srapath
  • srapath-orig
  • sratools
  • test-sra
  • vdb-config
  • vdb-copy
  • vdb-diff
  • vdb-dump
  • vdb-encrypt
  • vdb-lock
  • vdb-passwd
  • vdb-unlock
  • vdb-validate

Link to section 'Module' of 'sra-tools' Module

You can load the modules by:

module load sra-tools

Link to section 'Configuring SRA-Toolkit' of 'sra-tools' Configuring SRA-Toolkit

Users can config SRA-Toolkit by the command vdb-config. For example, the below command set up the current working directory for downloading:

vdb-config --prefetch-to-cwd

Link to section 'Example job' of 'sra-tools' Example job

To run SRA-Toolkit on our cluster:

#!/bin/bash
#SBATCH -A myallocation     # Allocation name 
#SBATCH -t 1:00:00
#SBATCH -N 1
#SBATCH -n 8
#SBATCH --job-name=SRA-Toolkit
#SBATCH --mail-type=FAIL,BEGIN,END
#SBATCH --error=%x-%J-%u.err
#SBATCH --output=%x-%J-%u.out

module --force purge
ml sra-tools

vdb-config --prefetch-to-cwd # The data will be downloaded to the current working directory.  
prefetch SRR11941281
fastq-dump --split-3 SRR11941281/SRR11941281.sra
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